IVT circRNA

Circular RNA (circRNA) is a covalently closed single-stranded RNA species that does not require capping or a poly(A) tail and exhibits prolonged transgene expression compared to linear mRNA due to altered degradation kinetics. With these favorable characteristics, IVT circRNA is rapidly being developed for therapeutic applications including vaccines, treating genetic disorders, and modulating the immune system. VectorBuilder specializes in custom circRNA production at a variety of scales.

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Highlights

Proprietary sequence optimization for coding region and IRES

Experts design and production team for delivering circRNA with high yield, purity, and expression

Comprehensive QC Panel

Offering Details

Premade circRNA products
Therapeutic RNA Development: IVT vector design & cloning, IVT RNA production & purification.
LNP Encapsulation: standard and custom formulations, antibody-conjugated LNP.
CDMO Services: process development, GMP manufacturing, fill/finish.

Technical Information

circRNA cyclization and translation

VectorBuilder has developed an optimized production platform for the generation of high-yield and high-purity circRNA based on the Group I permuted intron-exon (PIE) self-splicing technology (Figure 1). The generation of circRNA by this mechanism results in minimal impurities and scar sequence. The translation of circRNA is IRES-dependent. The IRES recruits the ribosome to initiate translation internally on a transcript independent of its 5' end.

Figure 1. Mechanism of circRNA self-splicing and generation in vitro. The translation of circRNA is IRES dependent.

Experimental data

Figure 2. circRNA encoding EGFP was transfected into HEK293T cells and exhibited long-term expression compared to traditional mRNA.

Try our Premade IRES-EGFP IVT circRNA

Comparison of different types of IVT RNA

	mRNA	circRNA	saRNA
Structure	Linear; usually contains 5’ cap, 5’ UTR, ORF for GOI, 3’ UTR, and poly(A) tail	Circular; usually contains IRES and ORF for GOI	Linear; usually contains 5’ UTR, ORFs for replicase genes and GOI, 3’ UTR, and poly(A) tail
Cap	Requires 5’ Cap for stability and ribosome recruitment	No Cap; relies on IRES for ribosome recruitment	Requires 5’ Cap for stability and ribosome recruitment
RNA length (nt)	100 ~ 10,000	1000 ～ 5000	7000 ~ 10,000
Stability	Low	High	Low
Can modified nucleotides be added in production?	Yes	No	Yes
Expression level	Low	Medium	High
Expression duration	Short	Medium	Long
Immunogenicity	Low	Medium	High

Documents

Brochures & Flyers

User Instructions
Certificate of Analysis (COA)
Material Safety Data Sheet (MSDS)