HiExpress™ Firefly Luciferase LNP-mRNA

Ordering Information Price Match

Click here to view the amino acid sequence translated from HiExpress^TM Firefly Luciferease mRNA >>

Shipping and storage

Our LNP-mRNA products are stored in Tris buffer (pH 7.4) and can be stored at -80°C for up to 6 months. The products are shipped on dry ice and should be avoided for freeze-thaw cycles.

Experimental Validation

Figure 1. Firefly Luciferase IVT mRNA expression in vitro and in vivo. (A) Expression of HiExpress™ Firefly Luciferase mRNA and other luciferase mRNA in HEK293T cells. Cells grown on a 12-well plate were transfected with 0.5 ug of mRNA per well and luciferase activity was measured at 6 h, 24 h, 48 h, and 72 h post transfection. (B) Luciferase activity measured in adult C57BL/6 mice injected intramuscularly with 30 ug of LNP encapsulated mRNA at 6 h, 24 h, and 48 h post injection. FLuc WT indicates wild-type firefly luciferase. FLuc WT (co) indicates codon-optimized wild-type firefly luciferase. FLuc2 indicates Luc2 firefly luciferase.

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